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M9460658.TXT
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1994-06-25
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Document 0658
DOCN M9460658
TI Brain viral burden in HIV infection.
DT 9408
AU Achim CL; Wang R; Miners DK; Wiley CA; Department of Pathology,
University of California at San Diego,; La Jolla.
SO J Neuropathol Exp Neurol. 1994 May;53(3):284-94. Unique Identifier :
AIDSLINE MED/94231211
AB We quantitated the brain viral burden in autopsy material from AIDS
patients with and without HIV encephalitis. Central nervous system (CNS)
samples from 45 AIDS autopsies with less than 48 hours postmortem
autolysis and without significant non-viral opportunistic infections
were analyzed using immunocytochemistry (ICC), antigen capture assay
(ACA) and polymerase chain reaction (PCR). Approximately three-quarters
of the cases contained HIV DNA by PCR. The majority of these had
abundant gp41 detected by ICC, but approximately one-third had no HIV
p24 detected by ACA. With all assays, HIV proteins and DNA were most
abundant in deep gray matter. Approximately one-quarter of the cases
contained HIV p24 by ACA in both CNS tissue and cerebrospinal fluid. In
more than half of the cases cytomegalovirus was detectable in the brain
by PCR, however, only in the basal ganglia of one case was human herpes
virus-6 detectable by PCR. In conclusion, HIV infection of the CNS was
observed in the majority of AIDS autopsies, however, the quantity of
virus was variable between cases and within different neuroanatomical
regions. Differences between the quantitation methods could be either
technical or biological, however, any of them could be used to compare
assessment of HIV burden by different laboratories.
DE Base Sequence Brain/*MICROBIOLOGY/PATHOLOGY Cytomegalovirus/*ISOLATION
& PURIF Cytomegalovirus Infections/COMPLICATIONS/GENETICS/MICROBIOLOGY
DNA, Viral/ANALYSIS Encephalitis/ETIOLOGY/MICROBIOLOGY/PATHOLOGY
Herpesviridae Infections/COMPLICATIONS/GENETICS/MICROBIOLOGY
Herpesvirus 6, Human/*ISOLATION & PURIF Human HIV Core Protein
p24/ANALYSIS HIV Envelope Protein gp41/ANALYSIS HIV
Infections/COMPLICATIONS/GENETICS/IMMUNOLOGY/*MICROBIOLOGY/ PATHOLOGY
Immunohistochemistry Molecular Sequence Data Polymerase Chain Reaction
Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).